How to perform Antigen Retrieval (AR) in Immunohistochemistry (IHC)

抗原修复是指石蜡、冰冻、火棉胶、塑料切片免疫组织化学（IHC）前用胰蛋白酶、尿素、表面活性剂、微波缓冲液和金属盐等，使被掩盖的抗原决定簇或变性的抗原重新暴露或抗原性得到一定程度的恢复过程。 抗原修复能最大程度地恢复在制片等过程中损失的抗原性，使原来认为不能在石蜡切片上进行IHC的许多抗体获得了良好的染色结果，成为一种有效的补救措施。 本文的目的是概述AR-HIC的发展、应用和标准化。 一、AR技术. 加热AR技术. 微波加热方法. 在传统标准方法,经脱蜡、脱水和用3%H2O2阻断内源性过氧 (化)物酶，石

Total retrieval time of time of 45 minutes at 75-80 degrees celcius for nuclear antigen such as ki-67, ER, PR , etc. A must for cell line preps and delicate tissues such as brain , bone marrow and animal tissues.

Antigen retrieval is a non-enzymatic pretreatment for immunostaining to reduce or eliminate the chemical modifications caused by formalin fixation, through high temperature heating or strong alkaline solution (non-heating).

Learn how to perform heat-induced or enzymatic antigen retrieval for IHC with our detailed IHC antigen retrieval protocol.

IHC 抗原修復方法介紹 (IHC Antigen Retrieval) IHC實驗抗原修復的兩種方法：加熱法（也稱為熱誘導表位修復或 HIER）和酵素法。

Antigen retrieval is one of the most critical steps, and getting it right is the key to unlocking clear, reproducible data. In this guide, we'll share our expertise on why it matters and how to perform it correctly.

Learn more about antigen or epitope retrieval methods for immunohistochemistry (IHC) including de-paraffinizing and rehydrating.